ISBIO 2015 edition: from 13th to 19th July

Applications are closed

Instruct announcement

CM1306 Applicants (PhD students and young Post-docs) should follow the indications in site website for registration.  In the registration form please indicate that you are a COST CM1306 applicant in the field "motivation to attend the course”.

The study of protein-ligand interactions is fundamental to the understanding of biological systems and is at the heart of the drug discovery process. There are many biophysical methods available to study protein-ligand interactions that span from calorimetric techniques to spectroscopic and structural methods, including modeling. Young researchers are usually familiar with one or more of these methods but often do not have the opportunity to address the problem from an integrated structural biology perspective.

The second edition of this course is stimulated by the high number of applicants of its first edition (October 6-12, 2014)  and builds on the experience gained by the tutors and the extremely positive feedback of the participants, namely that the course would have an impact in their research.

Therefore our main objective is not only to continue to illustrate but also to reinforce the added value of a structural biology approach to the study of protein-ligand interactions. In this edition this will be achieved by introducing new core methodologies; EPR and Molecular Modeling, to be combined with the 4 of the first edition, X-ray Crystallography, NMR, Carbohydrate Microarrays and ITC, always explored from a hands-on perspective, giving emphasis to their limitations and complementarities.

To achieve this, and to better illustrate the integrative context, the students will receive training by working in a study-case, a protein that recognizes and binds to carbohydrates, which will be the focus of the several complementary methodologies. Carbohydrate Microarrays will identify candidate ligands and analyze the carbohydrate-binding specificity; ITC will evaluate the affinity and thermodynamics of the protein-carbohydrate interaction; NMR studies will include protein-ligand interaction studies with the identified carbohydrate ligands using protein chemical shift perturbation and the ligand observed STD-NMR technique. X-Ray Crystallography methods, more specifically Molecular Replacement or simple difference Fourier, will be used to solve the 3D structure of the protein-carbohydrate complexes and derive the molecular determinants of the interaction. Molecular Modeling will encompass modern theoretical methods and computational techniques used to study protein-ligand interactions and the complementarity to the other experimental techniques will be explored.

Besides the hands-on training there will be theory lectures where the main theoretical aspects of each technique will be explained always with an emphasis on the complementarity with the other techniques.

You can download our poster.