DAY 1 - Monday 11th February 2013

Morning Session 

10:30 - 12:30

Introduction.

NMR in the context of protein interactions. Review of the fundamental parameters that can be used to study interactions, their scope and limitations. 

14:00 - 17:30

Worked examples.

Mapping interactions on structure based on chemical shift perturbation methods. The ligand viewpoint and the protein viewpoint.

DAY 2 - Tuesday 12th February 2013

Morning Session 

9:00 - 12:30

Concepts of relaxation in NMR. Relaxation and the study of dynamic processes.

Afternoon Session

14:00 - 17:30

Worked examples.

Analysis of relaxation and dynamics data.

DAY 3 - Wednesday 13th February 2013

Morning Session 

9:00 - 12:30

The Nuclear Overhauser Effect

Saturation Transfer Difference: Concepts and applications. 

The transfer NOE experiment.

Afternoon Session

14:00 - 17:30

The STD NMR experiment: setup, processing and analysis

 - determination of binding epitopes and KD

DAY 4 - Thursday 14th February 2013

Morning Session 

9:00 - 12:30

Information driven modelling of biomolecular complexes

Afternoon Session

14:00 - 17:30

hands on tutorials - protein-protein interactions

DAY 5 - Friday 15th February 2013

Morning Session 

9:00 - 12:30

hands on tutorials protein-ligand interactions

Afternoon Session

14:00 - 17:30

hands on tutorials protein-ligand interactions